Download DNA Fingerprinting: An Introduction (Breakthroughs in by Lorne T. Kirby PDF

By Lorne T. Kirby

DNA fingerprinting is a progressive procedure that permits scientists to check minute tissue samples and enables clinical stories at the composition, replica, and evolution of animal and plant populations. As a device for optimistic id of criminals, it performs a very vital function in forensic technology. the 1st ebook to be released within the box, , DNA Fingerprinting is a pragmatic consultant to uncomplicated ideas and laboratory equipment as utilized to various fields together with forensic research, paternity trying out, clinical diagnostics, animal and plant sciences, and flora and fauna poaching.

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Extra info for DNA Fingerprinting: An Introduction (Breakthroughs in Molecular Biology)

Sample text

S represents a unique DNA segment, Z represents a highly repetitive segment at a specific site, and F represents families of homologous segments found on multiple chromosomes. (4) 1,... is a sequential number that, in conjunction with the other three components, gives uniqueness to each segment. D4S139, for example, designates a specific locus on chromosome 4, and DYZ1 designates a specific highly repetitive sequence locus on the Y chromosome. REFERENCES Emery AE and Mueller RF. 1988. Elements of Medical Genetics.

Because of the many steps involved in recombinant DNA technology, including documentation, DNA isolation, digestion, electrophoresis, transfer, probe labeling, hybridization, autoradiography, interpretation, and confirmation of interpretation, at least two technologists should be available at all times. To accommodate holidays, sick days, and training time, at least two full-time individuals and one part-time person are required. The director of a newly established service laboratory must be very much in evidence during the initial months of operation to assess personnel.

Oogenesis in the human female is characterized by a primary oocyte undergoing meiosis to form one haploid ovum containing most of the cytoplasm and two nonviable polar bodies, which are discarded. The primary oocytes remain suspended in prophase I from the time of birth until sexual maturity (approximately 15 years). Meiotic divisions then resume overthe reproductive period, which may last 35 years. Linkage 19 Figure 2-11. Crossing over. Sister chromatids of homologous pairs of chromosomes exchange corresponding segments during meiosis I.

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