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Additional problems are encountered if supplements have certain characteristics such as a strong lipophily, as in the case of hydrocarbons, or if solid materials are used as the carbon and energy source. The objective of a medium composition study is twofold: -- to identify all components that are necessary for balanced growth or that may interfere with growth kinetics in some way: qualitative aspect. -- to establish the concentration of the components in an appropriate manner for the definition of the limiting substrate: quantitative aspect.

For bacteria [ (Schuegerl 5)), for yeasts (Suomala~nen and Oura 49)). Analytical data on dry matter, as discussed earlier, are also of great help for designing an initial usable composition. , COz). In essence, a defined medium is composed of a carbon and energy source, nutrients, trace elements and growth factors. STEP 2: Preparation of the medium Normally, the necessary medium components are dissolved in water arid mixed to make up the final growth solution. This method is acceptable as long as no chemical refictions lead to the formation of precipitates and no heat susceptible substances must be added.

19a and b. Chemostat experiments are excellent tools for testing the effects of chemical parameters. Biomass formation and residual glucose concentration plus respiratory quotient and mass (carbon) balance in function of the dilution rate are most informative for medium design. 1 (h- 1), the low biomass formation at low D and the unspecific RQ data lead to the conclusion that the medium was not carbort limited (Table l 3). Systematic medium design for this yeast yielded a regulatory pattern similar to that of Sacch.

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