By Arthur E. Humphrey (auth.), Professor Shintaro Furusaki, Dr. Isao Endo, Professor Ryuichi Matsuno (eds.)
Biochemical engineering types a bridge among basic biochemical examine and massive scale biotechnology techniques. It covers genetic and protein engineering, telephone tradition, bioprocess and reactor layout, separation and modelling. study paintings in biochemical engineering is an funding sooner or later, while traditional assets must get replaced with renewable ones. during this e-book the papers awarded on the Asia-Pacific Biochemical Engineering convention (Yokohama, Japan 1992) are amassed. This assortment is exclusive in its vast assurance of subject matters and it supplies an summary of the present developments of analysis in a tremendous area.
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Extra resources for Biochemical Engineering for 2001: Proceedings of Asia-Pacific Biochemical Engineering Conference 1992
B. brevis HPD31 carrying pNU200EGF produced as much as 240 mgll of hEGF. The produced hEGF had the same NH 2 -terminal sequence and biological activities as those of mature hEGF. pHY700EGF was constructed by inserting the 24 above fused gene into pHY700. B. 1 gil of hEGF (Table 1). brevis HPD31 carrying pHY700EGF Human salivary a-amylase This a-amylase consists of 511 amino acid residues, including 11 Cys residues, Hi th a molecular Height of about 56,000. The MWP signal peptide-encoding sequence Has directly fused Hi th the mature a -amylase-encoding sequence and then inserted into pHWl , a 101'1 copynumber plasmid of S.
2 (A) Immunoprecipitation of metabolically labeled transfectant clones. Antibodies used are M: rabbit anti-mouse IgM (Zymed); E: rabbit antihuman EGFR cytoplasmic domain. The clones were metabolically labeled with [35S 1methionine, lysed, divided into two and immunoprecipitated with either antibodies. Equal counts of TeA insoluble lysates were used per sample. (B) Autophosphorylation of affinity purified hybrid receptors. Samples are electrophoresed on SDS/5% PAGE at reduced or unreduced conditions.
Notable amongst these is the Report of the UK Council of Science and Technology, 'Developments in Biotechnology' (1990). Opportunities for research and commercialisation are categorised : 1 . High value chemicals, including a range of new human therapeutic products and diagnostics where recombinant DNA technology has allowed their successful large-scale production. 2. Intermediate value chemicals, including vitamins, drugs, pesticides and chemicals traditionally made by organic synthesis. Microbial biotransformations to produce biologically active isomers are of particular interest.